TY - JOUR
T1 - Structural Study of the Complex Between Human Pepsin and a Phosphorus-Containing Peptidic Transition-State Analog
AU - Hanson, John
AU - Fujinaga, Masao
AU - Cherney, Maia M.
AU - Tarasova, Nadya I.
AU - Bartlett, Paul A.
AU - James, Michael N. G.
AU - James, Michael C
N1 - Fujinaga, Masao, Maia M. Cherney, Nadya I. Tarasova, Paul A. Bartlett, John E. Hanson, and Michael N. G. James. "Structural Study of the Complex between Human Pepsin and a Phosphorus-Containing Peptidic Transition-State Analog." Acta Crystallographica Section D. 56.3 (2000): 272-279. Print.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - The refined crystal structure of the complex between human pepsin and a synthetic phosphonate inhibitor, Iva-Val-Val-Leu P -(O)Phe-Ala-Ala-OMe [Iva = isovaleryl, Leu P = the phosphinic acid analog of L-leucine, (O)Phe = L-3-phenyllactic acid, OMe = methyl ester], is presented. The structure was refined using diffraction data between 30 and 1.96 Å resolution to a final R factor (, where | F o | and | F c | are the observed and calculated structure-factor amplitudes, respectively) of 20.0%. The interactions of the inhibitor with the enzyme show the locations of the binding sites on the enzyme from S4 to S3'. Modeling of the inhibitor binding to porcine pepsin shows very similar binding sites, except at S4. Comparison of the complex structure with the structures of related inhibitors bound to penicillopepsin helps to rationalize the observed differences in the binding constants. The convergence of reaction mechanisms and geometries in different families of proteinases is also discussed.
AB - The refined crystal structure of the complex between human pepsin and a synthetic phosphonate inhibitor, Iva-Val-Val-Leu P -(O)Phe-Ala-Ala-OMe [Iva = isovaleryl, Leu P = the phosphinic acid analog of L-leucine, (O)Phe = L-3-phenyllactic acid, OMe = methyl ester], is presented. The structure was refined using diffraction data between 30 and 1.96 Å resolution to a final R factor (, where | F o | and | F c | are the observed and calculated structure-factor amplitudes, respectively) of 20.0%. The interactions of the inhibitor with the enzyme show the locations of the binding sites on the enzyme from S4 to S3'. Modeling of the inhibitor binding to porcine pepsin shows very similar binding sites, except at S4. Comparison of the complex structure with the structures of related inhibitors bound to penicillopepsin helps to rationalize the observed differences in the binding constants. The convergence of reaction mechanisms and geometries in different families of proteinases is also discussed.
KW - pepsins
KW - transition-state analogs
KW - phosphonate inhibitors
KW - proteinases
UR - https://soundideas.pugetsound.edu/faculty_pubs/1660
UR - http://dx.doi.org/10.1107/S0907444999016376
M3 - Article
JO - Acta Crystallographica Section D
JF - Acta Crystallographica Section D
ER -